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Lotte Knudsen joined the EEAS in 2016 as Managing Director responsible for Human Rights, global and multilateral issues, having previously served as Director for Human Development and Migration in the Directorate-General for International Cooperation and Development from 2014 – 2016.

Before that she was Director for "Criminal Justice" in the Justice Directorate-General from 2010 – 2014, and was Acting Director for Security in the same DG for two years.

Lotte Knudsen has worked in the Foreign Office of the UK and different services in the Commission, including the Secretariat General, External Relations including relations with the United States, enlargement and as an Assistant.